Skip to main navigation menu Skip to main content Skip to site footer
ACI Avances en Ciencias e Ingenierías

SECTION B: LIFE SCIENCES

Vol. 2 No. 2 (2010)

In vitro culture of mortiño (<em>Vaccinium floribundum</em> Kunth)

DOI
https://doi.org/10.18272/aci.v2i2.27
Submitted
July 2, 2015
Published
2010-06-01

Abstract

Due to the economic and agronomic potencial that mortiño (Vaccinium floribundum Kunth) has in Ecuador, the objective of this research was to establish in vitro propagation methods for this species by seed germination and by shoot growth from axillary buds. Modified Woody Plant Medium [1] (mWPM) was used, in which up to 60.7% of seeds germinated. A strong cytokine, trans-zeatin riboside, TZR (1 mg/l), combined with a-napthaleneacetic acid, NAA (0.05 mg/l), was used to subculture the in vitro germinated plantlets. The use of basal medium without hormones or with 2iP, 6-(gamma,gamma-dimethylalylamino) pu­rine, allowed elongation and rooting of plantletss. The acclimatization of mortiño plants has not yet been standardized, and further research is needed to find the conditions that are suitable for this process. To establish in vitro cultures by axillary buds, apical segments of the stem were disinfected and introduced in medium mWPM containing TZR, (7 mg/l) combined with NAA (0.1 mg/l). Growing buds were transferred to media with 2iP (3 or 5 mg/l) for propagation and elongation. This investigation is a preliminary study for the in vitro culture of mortiño, setting the basis for future research with this species.

viewed = 2491 times

References

  1. Lloyd, G. , and McCown, B . 1980 . "Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture." Comb. Proc. Intl PlantProp. Soc. 30,421-427.
  2. Asturizaga, A. S., 0llgaard, B., and Balslev, H. 2006. "Frutos comestibles." Botánica Económica de los Andes Centrales. 329-346.
  3. Luteyn, J. L. 2002. "Diversity, adaptation, and endemism in neotropical ericaceae: Biogeographical patterns in the Vaccinieae." The Botanical Review. 68, 55-87.
  4. Debnath, S. C. 2006. "Propagation of Vaccinium in vitro: A review." International Journal ofFruit Science. 6,4771.
  5. Grupo El Comercio (eds.) Noviembre 2008. "El morti-ño es un apetitoso regalo de la tierra de la serranía de Ecuador." Revista Líderes. 3, 17.
  6. Taji, Acram, P. K. and Lackshmanan, P. 2002. In vitro Plant Breeding. 1-4.
  7. Debnath, S. C. 2004. "In vitro culture of lowbush blueberry (Vaccinium angustifolium Ait.)" Small Fruits Review. 39, 393-408.
  8. Debnath, S. C. and McRae, K. B. 2001. "In vitro culture of lingonberry (Vaccinium vitis-idaea l.): The influence of cytokinins and media types on propagation." Small Fruits Review. 1,3-19.
  9. Ostrolucká, M., Libiaková, G., Ondrusková, E., and Gaj-dosová, A. 2004. "In vitro propagation of Vaccinium species." Acta Universitatis Latviensis, Biology. 676, 207212.
  10. Debnath, S. C. 2003. "Improved shoot organogenesis from hypocotyl segments of lingonberry (Vaccinium vitis-idaea l.)"In Vitro Cell. Dev. Biol. 39, 490-495.
  11. Pereira, J. M. 2006. "Conservation of Vaccinium cylin-draceum Smith (Ericaceae) by micropropagation using seedling nodal explants." In Vitro Cell. Dev. Biol. 42, 65-68.
  12. Jaakola, L., Tolvanen, A., Laine, K. and Hohtola A. 2001. "Effect of N6-isopentenyladenine concentration on growth initiation in vitro and rooting of bilberry and lingonberry microshoots." Plant Cell Tissue and Organ Culture. 66, 73-77.
  13. Murashige, T. and Skoog., F. 1962. "A revised medium for rapid growth and bioassay with tobacco tissue cultu-res."Physiol. Plantarum. 15,473-497.
  14. Rowland, L. J. and Ogden, E. L. 1992. "Use of a cy-tokinin conjugate for efficient shoot regeneration from leaf sections of highbush blueberry." HortScience. 27, 1127-1129.
  15. González, M. V, López, M., Valdez, A. E., and Ordas, R. J. 2000. "Micropropagation of three berry fruit species using nodal segments from fieldgrown plants." Ann. appl Biol. 137,073-078.
  16. Tetsumura, T., Matsumoto, Y., Sato, M., Honsho, C., Yamashita, K., Komatsu, H., Sugimoto, Y, and Kunitake, H. 2008. "Evaluation of basal media for micropropagation of four highbush blueberry cultivars." Scientia Hor-ticulturae. 1-3.
  17. Cao, X., Fordham, I., Douglass, L., and Hammerschlag, F. A. 2003. "Sucrose level influences micropropagation and gene delivery into leaves from in vitro propagated highbush blueberry shoots." Plant Cell Tissue and Organ Culture. 75, 255-259.
  18. Taiz, L. and Zeiger, E. 1998. Plant Physiology. Sunderland, Ma. Sinauer Associates, Inc. Publishers. 483-516.
  19. Debnath, S. C. and McRae, K. B. 2001. "An efficient in vitro shoot propagation of cranberry (Vaccinium macro-carpon Ait.) by axillary bud proliferation." In Vitro Cell. Dev. Biol. 37, 243-249.
  20. Reed, B. M. and Abdelnour-Esquivel, A. 1991. "The use of zeatin to initiate in vitro cultures of Vaccinium species and cultivars." HortScience. 26, 1320-1322.
  21. Eccher, T. N., Noé, N. 1989. "Comparison between 2ip and zeatin in the micropropagation of highbush blueberry (Vaccinium corymbosum)." Acta Horticulturae. 241, 185-190.
  22. Noé, N., Eccher, T. N. 1994. "Influence of irradiance on in vitro growth and proliferation of Vaccinium corym-bosum (highbush blueberry) and subsequent rooting in vivo." Physiologia Plantarum. 91, 273-275.

Most read articles by the same author(s)

1 2 > >>