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ACI Avances en Ciencias e Ingenierías

SECTION B: LIFE SCIENCES

Vol. 6 No. 1 (2014)

Characterization of lactic acid bacteria isolated from traditional Ecuadorian fermented foods

DOI
https://doi.org/10.18272/aci.v6i1.155
Submitted
September 29, 2015
Published
2014-06-13

Abstract

Ninety one samples of traditional fermented foods were collected from 20 Ecuadorian provinces. In order to identify strains with potential use as starter cultures in fermented foods, 119 lactic acid bacteria (LAB) strains were isolated and characterized by phenotypic tests and 16S ribosomal DNA (rDNA) sequencing. Forty seven percent of isolates were identified as facultative heterofermentative Lactobacillus sp. the next predominant groups (31%) were homofermentative cocci belonging to the genera Pediococcus, Lactococcus and Enterococcus; Finally, 11% were obligate heterofermentative Lactobacillus sp. and 11% heterofermentative cocci (Weissella and Leuconostoc). Several isolates showed Exopolysaccharide and bacteriocin production as well as caseinolytic activity. These properties could have interesting applications in food industry.

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References

  1. Carr, F.; Chill, D.; Maida, N. 2002. "The lactic acid bacteria: A literature survey". Cr. Rev. Microbiol, 28: 281-370.
  2. Jin, Y.; Ai, H.; Cheng, J.; Wu, M. 2009. "First description of a novel Weissella species as an opportunistic pathogen for rainbow trout Oncorhynchus mykiss (Walbaum) in China". Vet. Microbiol, 136: 314-320.
  3. Jashbhai, B.; Prajapati, M.; Baboo, M. 2008. "The history of fermented foods". CRC Press Taylor & Francis Group. New York.
  4. Simango, C. 1997. "Potential use of traditional fermented foods for weaning in Zimbabwe". Soc. Sci. Med, 44: 1065-1068.
  5. Gadaga, T.; Mutukumira, A.; Narvhus, J.; Feresu, S. 1999. "A review of traditional fermented foods and beverages of Zimbabwe". Int. J. of Food Microbiol, 53: 1-11.
  6. Liong, M. 2008. "Safety of probiotics: Translocation and infection". Nutr. Rev, 66: 192-202.
  7. Tamang, J. 2007. "Fermented foods for human life". In Microbes for Human Life, International Publishing House Pvt. Limited.eds. New Delhi, India.
  8. Valyasevi, R.; Rolle, R. 2002. "An overview of small-scale food fermentation technologies in developing countries with special reference to Thailand: scope for their improvement". Int. J. of Food Microbiol, 25: 231-239.
  9. Hassa"™ine, O.; Zadi-Karam, H.; Karam, N. 2008. "Phenotypic identification and technological properties of lactic acid bacteria isolated from three breeds dromedary raw milks in south Algeria". EJFA, 20: 46-59.
  10. Hill, S.; Gasson, M. 1986. "A qualitative screening procedure for the detection of casein hydrolysis by bacteria, using sodium dodecyl sulphate polyacrylamide gel electrophoresis". J. Dairy Res, 53: 625-629.
  11. Laemmli, U.; Molbert, E.; Showe, M.; Kelenberger, E. 1970. "Form-determining function of genes required for the assembly of the head of bacteriophage T4". J. Mol. Biol, 49: 99-113.
  12. Mayeux, J.; Sandine, W.; Elliker, P. 1962. "A selective medium for detecting Leuconostoc organisms in mixed isolate starter cultures". J. Dairy Sci, 45: 655-656.
  13. Tagg, J.; McGiven, A. 1971. "Assay system for bacteriocins". Appl. Microbiol, 21: 943.
  14. Pandey, G.; Yoshikawa, K.; Hirasawa, T.; Nagahisa, K.; Katakura, Y. 2007. "Extracting the hidden features in saline osmotic tolerance in Saccharomyces cerevisiae from DNA microarray data using the self-organizing map: Biosynthesis of amino acids". Applied Microbiol. Biotechnol, 75: 415-426.
  15. Martin, F.; Philippot, L.; Hallet, S.; Chaussod, R.; Germon, J.; Soulas, G.; Catroux, G. 2001. "DNA extraction from soils: old bias for new microbial diversity analysis methods". Appl. Environ. Microbiol, 67: 2354-2359.
  16. Bonfield, J.; Staden, R. 1996. "Experiment files and their application during large scale sequencing projects". DNA Sequence, 6: 109-117.
  17. Thompson, J.; Higgins, D.; Gibson, T. 1994. "CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice". Nucleic Acids Res, 22: 4673-4680.
  18. Kumar, S.; Tamura, K.; Nei, M. 2004. "MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment". Brief Bioinform, 5: 150-163.
  19. Amoa-Awua, W.; Appoh, F.; Jakobsen, M. 1996. "Lactic acid fermentation of cassava dough into agbelima". Int. J. of Food Microbiol, 31: 87-98.
  20. Ben Omar, N.; Ampe, F.; Raimbault, M.; Guyot, J.; Tailliez,P. 2000. "Molecular diversity of lactic acid bacteria from cassava sour starch (Colombia)". Syst. Appl. Microbiol, 23: 285-291.
  21. Ngaba, P.; Lee, J. 1979. "A research note: fermentation of cassava (Manihot esculenta Crantz)". J. Food. Sci, 44: 1570-1571.
  22. Watabe, J.; Ikeda, N.; Mizutani, J.; Sato, N.; Jin, S.; Hirai, T.; Ariga, H. 1998. "Comparison of microbiological and chemical characteristics among types of traditionally fermented milk in Inner Mongolia in China and Calpis sour milk (Sannyuu)". Milk Sci, 47: 1-7.
  23. Daeschel, M.; Andersson, R.; Fleming, H. 1987. "Microbial ecology of fermenting plant materials". FEMS Microbiol. Rev, 46: 91-98.
  24. Kostinek, M.; Specht, I.; Edward, V; Pinto, C.; Egounlety, M.; Sossa, C.; Mbugua, S.; Dortu, C.; Thonart, P.; Taljaard, L.; Mengu, M.; Franz, M.; Holzapfel, W. 2007. "Characterization and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures". Int. J. Food Microbiol, 114: 342-351.
  25. Cai, Y.; Ohmomo, S.; Ogawa, M.; Kumai, S. 1999. "Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage". J. Dairy Sci, 82: 520-526.
  26. Rantsiou, K.; Urso, R.; Dolci, P.; Comi, G.; Cocolin, L. 2008. "Microflora of Feta cheese from four Greek manufacturers". Int. J. of Food Microbiol, 126: 36-42.
  27. Tzanetakis, N.; Litopoulou-Tzanetaki, E. 1992. "Changes in numbers and kinds of lactic acid bacteria in Feta and Teleme, two Greek cheeses from Ewes"™ milk". J. of Dairy Sci, 75: 1389-1393.
  28. Everson, C.; Danner, W.; Hammes, P. 1970. "Bacterial starters in sausage products". J. Agr. Food Chem, 18: 570-571.
  29. Everson, C.; Danner, W.; Hammes, P. 1970. "Improved starter culture for semidry sausage". Food Tech, 24: 42-44.
  30. Beukes, E.; Bester, B.; Mostert, J. 2001. "The microbiology of South African traditional fermented milks". Int. J. ofFoodMicrobiol, 63: 189-197.
  31. Bj"™orkroth, K.; Schillinger, U.; Geisen, R.; Weiss, N.; Hoste, B.; Holzapfel, W.; Korkeala, H.; Vandamme, P. 2002. "Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov., detected in food and clinical samples". Int. J. Syst. Evol. Micr, 52: 141-148.
  32. De Vuyst, L.; Vandamme, E. 1994. "Antimicrobial potential of lactic acid bacteria". In Bacteriocins of Lactic Acid Bacteria. Blackie Academic and Professional. UK.
  33. Salama, M.; Musafija-Jeknic, T.; Sandine, W.; Giovannoni, S. 1995. "An ecological study of lactic acid bacteria: isolation of new strains of Lactococcus including Lactococcus lactis subspecies cremoris". J. of Dairy Sci, 78: 1004-1017.
  34. Franz, C.; Holzapfel, W.; Stiles, M. 1999. "Enterococci at the crossroads of food safety". Int. J. of Food Microbiol, 47: 1-24.
  35. Holzapfel, W. 1997. "Use of starter cultures in fermentation on a household scale". Food Control, 8: 241-258.
  36. Axelsson, L. 1998. "Lactic acid bacteria: classification and physiology". In Salminen, S. Von Wright, A. (Eds.). Lactic Acid Bacteria: Microbiology and Functional Aspects. Marcel Dekker. New York,: 19-54.
  37. Christensen, J.; Dudley, E.; Pederson, J.; Steelz, L. 1999. "Peptidases and amino acid catabolism in lactic acid bacteria". Antonie van Leeuwenhoek, 76: 217-246.
  38. Bockelmann, W. 1995. "The proteolytic system of starter and non-starter bacteria: Components and their importance for cheese ripening". Int. Dairy J, 5: 977-994.
  39. Sánchez-Marroquín, A.; Larios, C.; Vierna, L. 1967. "Estudios sobre la microbiología del pulque XIX. Elaboración de la bebida mediante cultivos puros". Rev. Latinoam. Microbiol, 9: 83-85.
  40. Ulloa, M.; Herrera, T. 1976. "Estado actual del conocimiento sobre la microbiología de bebidas fermentadas indígenas de México: pozol, tesg"™uino, pulque, colonche y tepache". A. Inst. Biol. Mex, 47: 145-163.
  41. Kim, D.; Robyt, J. 2014. "Production, Selection and Characteristic of mutants of Leuconostoc mesenteroides B-742 constitutive for dextran". Enzyme Microb. Tech, 17: 689-695.
  42. Daeschel, M.; McKenney, M.; McDonald, L. 1990. "Bacteriocidal activity of Lactobacillus plantarum C11". Food Microbiol, 7: 91-98.
  43. West, C.; Warner, P. 1988. "Plantacin B, a bacteriocin produced by Lactobacillus plantarum NCDO 1193". FEMS Microbiol. Lett, 49: 163-165.
  44. Atrih, A.; Rekhif, N.; Milliere, J.; Levebvre, G. 1993. "Detection and characterization of a bacteriocin produced by Lactobacillus plantarum C19". Can. J. of Microbiol, 39: 1173-1179.
  45. Garriga, M.; Hugas, M.; Aymerich, T.; Monfort, J. 1993. "Bacteriocinogenic activity of lactobacilli from fermented sausages". J. Appl. Bacteriol, 75: 142-148.
  46. Herbert, C. 1957. "Rice Fermentation in Ecuador". Economic Botany, 11: 267-270.
  47. Van Veen, A.; Graham, D.; Steinkraus, K. 1968. "Fermented rice, a food from Ecuador". Arch. Latinoam. Nutr, 18: 363.
  48. Papalexandratou, Z.; Falony, G.; Romanens, E.; Jimenez, J.; Amores, F.; Daniel, H.; De Vuyst, L. 2011. "Species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional Ecuadorian spontaneous cocoa bean fermentations". Appl. Environ. Microbiol, 77: 7698-7714.

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