Keywords
PCR
DNA
Ecuador
How to Cite
Abstract
This report describes the first detection of Porcine Circovirus Type 2 (PCV2) from pigs in Ecuador. Samples were collected from 162 animals from November 2010 to March 2011: 25 sick animals from commercial swine herds and 137 from slaughterhouses. To detect the virus we used polymerase chain reaction (PCR) and two primer set. Our procedure revealed PCV2 DNA in 24 of 25 clinically affected pigs from farms and 77 of 137 pigs from slaughterhouses. The nucleotide sequence analysis of nine amplicons confirmed the PCV2 detection with at least a 95% homology compared with other PCV2 found in the GenBank database. Additionally, microscopic lesions in tissues of clinically affected pigs suggested porcine circovirus infection.
References
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