Keywords
horizontal gen transfer
antibiotic resistance genes
environmental bacteria
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Abstract
This study looked for the presence of environmental antibiotic resistance genes and their capacity to disseminate through conjugation. Water and soil samples were collected from pristine zones of the Ecuadorian Amazon Basin (Sucumbios, Napo and Orellana provinces), and they were inoculated in a modify wheat grain medium (WGM). Some of WGM cultures contained diverse bacterial species that were able to transfer antibiotic resistance genes to Escherichia coli K12. Finally, 10 strains were isolated and proved to be responsible of antibiotic resistance gene transfer. Strains were identified (using 16S rDNA sequences) as Serratia sp., Pseudomonas sp., Listonella sp., and Aeromonas sp. In this work, we proved that environmental bacteria can transfer antibiotic resistance genes as tetraciclin and ampicilin.
References
Allen, K. 2009. Functional metagenomics reveals diverse beta lactamases in a remote Alaskan soil. ISME Journal. 3,243-251.
Bustos, E. 1985. Transferencia plasmidial de multirre-sistencias en cepas enteropatogénicas de E. coli. Revista Chilena de Pediatría. 56, 445-449.
Correa, G. 2008. Impacto de la resistencia a los antibióticos en el desarrollo de la medicina contemporánea. Revista Médica. 16,9-10.
Gérvas, J. 2000. La resistencia a los antibióticos un problema de salud pública. Atención Primaria. 25, 589-596.
Hall, B. 2004. Predicting the evolution of antibiotic resistance genes. Nature. 2, 430-435.
Jorgen, J. 2005. Ribosomal DNA sequencing. APMIS. 113,621-628.
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