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SECTION B: LIFE SCIENCES

Vol. 4 No. 1 (2012)

In vitro evaluation of fungicides to control Monilinia spp. that affects the cultivation of peach (Prunus persica) in the provinces of Ecuadorian Sierra

DOI
https://doi.org/10.18272/aci.v4i1.80
Submitted
August 12, 2015

Abstract

Monilinia spp. is a phytopathogen that affects peach crops in the Ecuadorian highlands causing a disease known as brown rot. This disease represents a limiting factor in the crop"™s yield, producing losses of up to 50%. The aim of this study was to evaluate the efficiency of five commercial fungicides used for the control of Monilinia in 4 Ecuadorian provinces (Carchi, Imbabura, Pichincha and Tungurahua). For this purpose, fungi were isolated from peach fruits presenting the typical disease symptoms, and 21 samples were positively identified as Monilinia by morphological analysis (mycelium stain and spore shape). To evaluate the fungicide efficiency, the EC50 value (the effective fungicide concentration at which 50% of the conidial germination is inhibited) was determined using samples from Imbabura, Carchi and Tungurahua. It was found that the five fungicides tested (Bravo, Mertec, Rovral, Tilt and Switch) were very efficient in controlling this phytopathogen (EC50 <0.01 μg/ml). This research presents a useful approach for the establishment of strategies to control and prevent M. fructicola in peach cultivars and other crops that may be susceptible to this fungus in the country.

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References

  1. Layne, D. and Bassi, D. 2008. "The Peach: Botany, production and uses", Biddles: King's Lynn.
  2. Whealy, K., Vásquez, W., and Viteri, P. 2001. "Fruit berry and nut inventory", Third edition. United States.
  3. USDA: Foreing Agricultural Service. 2010. "Global agricultural information network". 2010-05-10. Consultado: 2010-11-06. <http://gain.fas.usda.gov>.
  4. SIGAGRO 2010. Ministerio de Agricultura, Ganadería Acuacultura y Pesca, MAGAP.
  5. Agrios, G. 1995. "Fitopatología", Segunda Edición. Uteha Noriega Editores: México.
  6. Coté, M., Tardif, M., and Meldrum, A. 2004. "Identification of Monilinia fructigena, M. fructicola, M. laxa and Monilinia polystroma on inoculated and naturally infected fruit using multiplex PCR". Plant Disease. 88, 1219-1225.
  7. Thomson, W. 1994. "Agricultural Chemical Book IV - fungicides", Fresno.
  8. Gregorí, B. 2005. "Estructura y actividad de los antifúngicos. Instituto cubado de investigaciones de derivados de la caña de azúcar". Rev. Cubana Farm. 39 (2).
  9. Borja, E. 2011. "Evaluación in vitro de fungicidas para el control de Monilinia spp. aislada de duraznos (Prunus persica) de diferentes localidades de la sierra del Ecuador". Universidad San Francisco de Quito, Tesis de Ingeniería: Quito.
  10. Salgado, C. 2011. "Identificación molecular de especies de Monilinia spp. que afectan la producción de durazno, Prunus persica, en las provincias de Carchi, Imbabura, Pichincha y Tungurahua". Universidad San Francisco de Quito, Tesis de Licenciatura: Quito.
  11. EPPO 2009. Diagnostic Protocol of Monilinia fructicola, European and Mediterranean Plant Protection Organization.
  12. Streets, R. 1982. "The diagnosis of plant diseases", Tucson: The University of Arizona.
  13. Cañedo, V and Ames, T. 2004. "Medios de cultivos de laboratorio: Manual de Laboratorio para el manejo de Hongos Entompatógenos". Perú: Centro Internacional de la papa (CIP). pp. 18-22.
  14. Everett, K. and Torrevilla, O. 2007. "In vitro fungicide testing for control of avocado fruit rots". New Zealand Plant Protection. 60, 99-103.
  15. Hartman, J. 2007. "Peach fruit diseases". Plant Pathology Fact Sheet: University of Kentucky, College of Agriculture.
  16. Gell, I., Cubero, J., and Melgarejo, P. 2007. "Two different pcr approaches for universal diagnosis of brown rot and identification of Monilinia spp. in stone fruit trees". Journal ofApplied Microbiology. pp. 1364-5072.
  17. Mitidieri, M., Constantino, A., Brambilla, J., Gabilondo, E., Parra, M., Veron, R., and Bimboni, G. 2005. "Effect of different early/season sprays on blossom blight incidence and yield in peach orchards". Acta Horticulturae 713: VI International Peach Symposium.
  18. INAMHI, boletín anual 2010.
  19. Obanor, F., Walter, M., Jones, E., and Jaspers, M. 2005. "In vitro effects of fungicides on conidium germination of Spilocacea oleagina, the cause of olive leaf spot". New Zealand Plant Pathology Protection Society. 58, 278-282.
  20. Adaskaveg, J.,Foster,H., Gubler, W., Teviotdale, B., and Thompson, D. 2005. "Reduced-risk fungicides help manage brown rot and other fungal disease of stone fruit". California agriculture. 59, 109-114.
  21. Jaspers, M. 2001. "Sesitivity of phaeomoniella chlamydospora to fungicides in vitro". New Zealand Plant Protection Society, Horticultural Pathology. 54, 225-228.
  22. Burpee, L. 1997. "Control of dollar spot of creeping bentgrass caused by an isolate of Sclerotinia homeocarpa resistant to benzimidazole and the demethylation - inhibitor fungicides". Plant Disease. 81, 1259-1263.
  23. Thomson, W. 1997. "Agricultural Chemicals. Book IV: Fungicides", Thomson Publications, Fresno, CA., 12 edition.
  24. Grindle, M. 1981. "Variations among field isolates of Botrytis cinerea in their sensitivity to antifungal compounds". Pesticide Science. 12, 305-312.
  25. Hermann, D. and Gisi, U. 1994. "Cross-resistance among dmi fungicides and sensitivity distributions of Septoria tritici populations". Brighton Crop Prot. Conf. - Pests and Diseases. 1, 487-492.
  26. Liggit, J., Jenkinson, P., and Parry, D. 1997. "The role of saprophytic microflora in the development of fusarium ear blight of winter wheat caused by Fusarium".
  27. Hilber, U. and Schuepp, H. 1996. "A reliable method for testing sensitivity to Botryotinia fuckeliana to anilopyrimidines in vitro". Pesticide Science. 47, 241-247.
  28. Parker, K. and Sutton, T. 1993. "Effect of temperature and wetness duration on apple fruit infection and eradicant activity of fungicides against Botryosphaeria dothidea". Plant Disease. 77, 181-185.