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ACI Avances en Ciencias e Ingenierías

SECTION B: LIFE SCIENCES

Vol. 5 No. 1 (2013)

Genetic diversity of an entomopathogenic fungi collection from Ecuador using a modified AFLP approach

DOI
https://doi.org/10.18272/aci.v5i1.121
Submitted
September 29, 2015
Published
2013-04-08

Abstract

The genetic diversity of 41 Ecuadorian entomopathogenic fungal strains plus one isolate from the USA, from a collection maintained by INIAP (Instituto Nacional Autónomo de Investigaciones Agropecuarias) was determined using a modified AFLP (Amplified Fragment Length Polymorphism) approach. We found genetic similarity indexes lower than 0.50 and 121 polymorphic bands. The AMOVA analysis revealed that between-group and within-group variation contributed in similar amounts (59% and 41%, respectively) to the whole genetic variation detected. The dendrogram built using Jaccard's genetic coefficient shows twelve groups, where seven of them contain isolates clustered by genus. From these seven groups, five of them contain isolates clustered by the host from which they were recovered. The Bootstrap values show twelve reliable phylogenetic relations with values higher than 70% of confidence. The Principal Components Analysis (PCA) produced six clusters; four of them contain isolates associated by genus. The results suggest the existence of a considerable genetic diversity within the INIAP's entopathogenic fungi collection, and a clustering tendency related to the host from which they were isolated. We did not find common genomic regions among the most virulent entomopathogenic fungi strains. The high genetic diversity found within this collection represents a potential source of genotypes with potent bioinsecticide activity.

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References

  1. Aquino de Muro, M.; Mehta, S.; Moore, D. 2003. "The use of amplified fragment length polymorphism for molecular analysis of Beauveria bassiana isolates from Kenya and other countries, and their correlation with host and geographical origin". FEMS Microbiology letters, 229: 249-257.
  2. Zimmermann, G. 2007. "Review on safety of the entomophatogenic fungus Beauveria bassiana and Beauveria brongniartii". Biocontrol Science and Technology, 17 (5/6): 553-596.
  3. Zimmermann, G. 2007. "Review on safety of the entomophatogenic fungus Metarhizium anisopliae". Biocontrol Science and Technology, 17(9): 879-920.
  4. Wang, L.; Huang, J.; You, M.; Guan, X.; B., L. 2005. "Effects of toxins from two strains of Verticillium lecanii (Hyphomycetes) on bioattributes of a predatory ladybeetle, Delphastus catalinae (Col., Coccinellidae)". JEN, 129(1): 32-38.
  5. Zengzhi, L.; Alves, S.; Roberts, D.; Meizhen, F.; Delalibera Jr., I.; Jian-Tang, L.; Rogério, B.; Faria, M.; Rangel, D. 2010. "Biological control of insects in Brazil and China: history, current programs and reasons for their successes using entomopathogenic fungi. ". Biocontrol Science and Technology, 20(2): 117-136.
  6. Mitina, G.; Mikhailova, L.; Yli-Mattila, T. 2008. "RAPD-PCR, UP-PCR and rDNA sequence analyses of the entomopathogenic fungus Verticillium lecanii and its pathogenicity towards insects and phytopathogenic fungi". Archives of Phytopathology and Plant Protection, 41(2).
  7. Poprawski, T.; Riba, G.; Jones, W.; Aioun, A. 1989. "Variation in isoesterase profiles of geographical populations of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolated from Sitona weevils (Coleoptera: Curculionidae)". Environmental Entomology, 17: 275-279.
  8. Magnai, L.; Bridge, P.; Evans, H. 1989. "A chemotaxonomic evaluation of the genus Beauveria. Mycological Research 92: 199-209. Neuveglise, C., Brygoo, Y., Vercambre, B., Riba, G. 1994. Comparative analysis of molecular and biological characteristics of strains of Beauveria brongniartii isolated from insects". Mycological Research, 98: 322-328.
  9. Neuveglise, C.; Brygoo, Y.; Vercambre, B.; Riba, G. 1994. "Comparative analysis of molecular and biological characteristics of strains of Beauveria brongniartii isolated from insects". Mycological Research, 98: 322-328.
  10. Viaud, M.; Couteaudier, Y.; Levis, C.; Riba, G. 1996. "Genome organization in Beauveria bassiana: electrophoretic karyotype, gene mapping, and telomeric fingerprint". Fungal Genetics and Biology, 20: 175-183.
  11. Cravanzola, F.; Piatii, P; Bridge, P; Ozino, O. 1997. "Detection of genetic polymorphism by RAPD-PCR in strains ofthe entomopathogeic fungus Beauveria brongniartii isolated from the European cockchafer (Melolontha spp.)". Letters in Applied Microbiology, 25: 289-294.
  12. Landázuri, P 2003. "Prospección y Evaluación de cepas de Beauveria sp. y Metarhizum sp. en las provincias de Carchi, Cotopaxi, Chimborazo y Cañar, para el control biológico de Gusano Blanco de la papa (Premnotrypes vorax)". Instituto de Postgrado-Escuela Politécnica del Ejército. Sangolquí-Ecuador.
  13. Barriga, E. 2003. "Evaluación de la Patogenicidad y Multiplicación en sustratos de aislamientos de Beauveria brogniartii y Metarhizum anisopliae para el control de Premnotrypes vorax en el laboratorio y campo". Facultad de Ciencias Agrícolas-Universidad Central del Ecuador.
  14. Valverde, I. 2004. "Aislamiento e Identificación de Hongos Entomopatógenos y pruebas de patogenicidad en Cosmopolitas sordidus y Metamasius hemipterus en condiciones de Laboratorio: San Cristóbal-Galápagos". Facultad de Ciencias Agrícolas - Universidad Central del Ecuador. Quito "” Ecuador.
  15. Rueda, M. 2005. "Prospección de Hongos Entomopatógenos para Macrodactylus pulchripes Blanchard (Aguacuro del Maíz), y su comportamiento de ovipostura: san José de Minas, Pichincha. Facultad de Ciencias Agrícolas". Universidad Central del Ecuador. Quito "” Ecuador.
  16. Ayala, O. 2006. "Determinación de agresividad de Hongos Entomopatógenos para Macrodactylus sp. (Catzo del Maíz): Chillanes-Bolívar". Facultad de Ciencias Agrícolas-Universidad Central del Ecuador Quito-Ecuador.
  17. Shagai-Maroof, M.; Soliman, K.; Jorgensen, R.; Allard, R. 1984. "Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal locations and population dynamics". Proceedings of the National Academy of Sciences of the USA, 81: 8014-8018.
  18. Pfeifer, T.; Khachatourians, G. 1993. "Electrophoretic karyotype of the entomopathogenic deuteromycete Beauveria bassiana". Journal ofInvertebrate Pathology, 61(3): 231-235.
  19. Shimizu, S.; Y, A.; T., M. 1992. "Electrophoretic karyotype of Metarhizium anisopliae". Journal of Invertebrate Pathology, 60(2): 185-187.
  20. Benbouza, H.; Jacquemin, J.; Baudoin, J.; G., M. 2006. "Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels". Biotechnology, Agronomy, Society and Environment, 10: 77-81.
  21. Berretta, M.; Lecuona, R.; Zandomeni, R.; Grau, O. 1998. "Genotyping isolates of the entomopathogenic fungus Beauveria bassiana by RAPD with fluorescent labels". Journal of Invertebrate Pathology, 71: 145-150.
  22. Maurer, P.; Couteaudier, Y.; Girard, P.; Bridge, P.; Riba, G. 1997. "Genetic diversity of Beauveria bassiana and relatedness to host insect range". Mycological Research, 101: 159-164.
  23. Glare, T.; Inwood, A. 1998. "Morphological and genetic characterisation of Beauveria spp. from New Zealand". Mycological Research, 102(2): 250-256.
  24. Rehner, S.; Buckley, E. 2005. "A Beauveria phylogeny inferred from nuclear ITS and EF1-{alpha} sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs". Mycologia, 97: 84-98.
  25. St. Leger, R.; Allee, L.; May, B.; Staples, R.; Roberts, D. 1992. "World-wide distribution of genetic variation among isolates of Beauveria spp". Mycological Research, 96: 1007-1015.
  26. Bidochka, M.; McDonald, M.; St. Leger, R.; Roberts, D. 1994. "Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic ADN (RAPD)". Current Genetics, 25: 107-113.
  27. Urtz, B.; Rice, W. 1997. "RAPD-PCR characterization of Beauveria bassiana isolates from the rice water weevil Lissorhoptrus oryzophilus". Letters in Applied Microbiology, 25: 405-409.
  28. Coates, B.; Hellmich, R.; Lewis, L. 2002. "Allelic variation of a Beauveria bassiana (Ascomycota: Hypocreales) minisatellite is independent of host range and geographic origin". Genome, 45: 125-132.
  29. Piatti, P.; Cravanzola, F.; Bridge, P.; Ozino, O. 1998. "Molecular characterization of Beauveria brongniartii isolates obtained from Melolontha melolontha in Valle d"™Aosta (Italy) by RAPD-PCR". Letters in Applied Microbiology, 26: 317-324.
  30. St. Leger, R.; Screen, S.; Shams, P. 2000. "Lack of host specialization in Aspergillus flavus". Applied and Environmental Microbiology, 66: 320-324.
  31. Devi, K.; Reineke, A.; Nageswara Rao Reddy, N.; Maheswara Rao, C.; Padmavathi, J. 2006. "Genetic diversity, reproductive biology, and speciation in the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin". Genome, 49: 495-504.