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ACI Avances en Ciencias e Ingenierías

SECTION B: LIFE SCIENCES

Vol. 14 No. 1 (2022)

Endophyte microbiome characterization in banana leaf and its variation in presence of Black Sigatoka pathogen (Pseudocercospora fijiensis)

DOI
https://doi.org/10.18272/aci.v14i1.2299
Submitted
May 15, 2021
Published
2022-05-12

Abstract

We describe the bacterial and fungal microbiome of the banana (Musa x paradisiaca) leaf in healthy and necrotic phases of Black Sigatoka or black leaf streak disease (Pseudocercospora fijiensis), evaluated under organic and conventional agronomic management in El Oro province, Ecuador. Samples were collected for subsequent DNA sequencing and analysis of the 16S (V3-V4) and ITS markers. This study suggests that the fungal microbiome from the organic farming system decreases its diversity due to pathogen's presence while diversity increases upon conventional practices. Also, we observed that bacteria from the genus Pseudomonas fluorescens was more abundant in the healthy organic leaf, a known beneficial microorganism for plants. The banana"™s phyllosphere endophyte microbiome depends on the cultivation system, and diseased conditions significantly change the microbiome in the presence of black leaf streak disease.

Key words: necrotic, sequencing, diversity, ASV, phyllosphere

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