Ir al menú de navegación principal Ir al contenido principal Ir al pie de página del sitio

SECCIÓN B: CIENCIAS BIOLÓGICAS Y AMBIENTALES

Vol. 14 Núm. 2 (2022)

Caracterización del microbioma de plantas de banano (Musa × paradisiaca L.) bajo sistemas de producción orgánico y convencional

DOI
https://doi.org/10.18272/aci.v14i2.2298
Enviado
mayo 15, 2021
Publicado
2022-12-12

Resumen

El cultivo de banano (Musa × paradisiaca L.) es una de las actividades agrícolas más importantes para muchos países y es la principal fruta consumida mundialmente. La fertilización química es una de las prácticas más comunes utilizada para aumentar el rendimiento y productividad de los cultivos. Una gran cantidad de literatura examina los cambios en las propiedades del suelo asociados con diferentes regímenes de fertilización. A pesar del papel fundamental del microbioma en la salud y la productividad de las plantas, los efectos de los diferentes sistemas de gestión agrícola sobre las comunidades microbianas son poco estudiados. Este estudio informa la estructura, diversidad y riqueza de la comunidad microbiana del suelo, rizosfera y hoja de las plantas de banano bajo manejo orgánico y convencional. Se obtuvieron muestras de dos plantaciones bananeras ubicadas en El Oro, una de las provincias con mayor productividad bananera en Ecuador. El análisis se basó en la secuenciación de ADN de la región V3-V4 del gen del ARNr 16S para bacterias y la región ITS2 para hongos. Se encontró un efecto significativo del sistema de manejo en la composición de la comunidad bacteriana y fúngica. En términos generales, bajo un sistema de manejo convencional, la riqueza y uniformidad de la comunidad bacteriana y fúngica aumentó entre las muestras de suelo y rizosfera en comparación con el sistema de agricultura orgánica. El suelo y la rizosfera bajo agricultura orgánica se asociaron con una mayor abundancia relativa de Proteobacteria, Firmicutes y Bacteroidetes y exhibieron una sobrerrepresentación de géneros microbianos conocidos como promotores del crecimiento de plantas, así como géneros involucrados en procesos importantes del ecosistema. También encontramos que los ASV del mismo género responden diferente a los dos tipos de manejo agrícola en el suelo y la rizosfera. Mientras que las comunidades bacterianas en las hojas fueron más similares en ambos tipos de manejo. Comprender cómo los sistemas de gestión de cultivos a largo plazo modifican la diversidad y la estructura microbiana, como se presenta en esta investigación, puede ayudar a diseñar sistemas agrícolas que puedan mantener una alta rentabilidad de los cultivos de banano mediante la estimulación de bacterias promotoras del crecimiento y supresoras de las enfermedades transmitidas por el suelo.

viewed = 103 times

Citas

FAO. (2017). El futuro de la alimentación y agricultura: tendencias y desafíos (I6881ES/1/02.17) http://www.fao.org/3/i6881s/i6881s.pdf

Khush, G. (1999). Green revolution: preparing for the 21st century. Genome, 42(4), 646-655. doi: https://doi.org/10.1139/g99-044

Paul, J., Choudhary, A., Suri, V., Sharma, A., Kumar, V. & Shobhna (2014). Bioresource nutrient recycling and its relationship with biofertility indicators of soil health and nutrient dynamics in rice-wheat cropping system. Communications in soil science and plant analysis, 45(7), 912-924. doi: https://doi.org/10.1080/00103624.2013.867051

FAO. (2020). FAOSTAT. Recuperado el 11 abril del 2020, desde: http://www.fao.org/faostat/es/#data

INEC. (2020). Encuesta de Superficie y Producción Agropecuaria Continua- ESPAC 2019. https://www.ecuadorencifras.gob.ec/documentos/web-inec/Estadisticas_agropecuarias/espac/espac-2019/Presentacion%20de%20los%20principales%20resultados%20ESPAC%202019.pdf

Marin, D., Romero, R., Guzmán, M & Sutton, T. (2003). Black Sigatoka: an increasing threat to banana cultivation. Plant disease, 87(3), 208-222. doi: https://doi.org/10.1094/PDIS.2003.87.3.208

Arvanitoyannis, I. & Mavromatis, A. (2009). Banana cultivars, cultivation practices, and physicochemical properties. Critical Reviews in Food Science and Nutrition, 49(2), 113-135.doi: https://doi.org/10.1080/10408390701764344

Gruber, N. y Galloway, J. (2008). An Earth-system perspective of the global nitrogen cycle. Nature, 451(7176), 293-296. doi: https://doi.org/10.1038/nature06592

Yin, C., Fan, F., Song, A., Li, Z., Yu, W. & Liang, Y. (2014). Different denitrification potential of aquic brown soil in Northeast China under inorganic and organic fertilization accompanied by distinct changes of nirS-and nirK-denitrifying bacterial community. European journal of soil biology, 65, 47-56. doi: https://doi.org/10.1016/j.ejsobi.2014.09.003

Evenson, R. y Gollin, D. (2003). Assessing the impact of the Green Revolution, 1960 to 2000. science, 300(5620), 758-762.

Oportunidades y desafíos del mercado internacional para el banano orgánico (2020, Agosto 12). Agrocalidad. https://www.agrocalidad.gob.ec/oportunidades-y-desafios-del-mercado-internacional-para-el-banano-organico/

Bahram, M., Hildebrand, F., Forslund, S., Anderson, J., Soudzilovskaia, N., Bodegom, P., & Bork, P. (2018). Structure and function of the global topsoil microbiome. Nature, 560(7717), 233-237. doi: https://doi.org/10.1038/s41586-018-0386-6

Bardgett, R. & Van Der Putten, W. (2014). Belowground biodiversity and ecosystem functioning. Nature, 515(7528), 505-511. doi: https://doi.org/10.1038/nature13855

Pershina, E., Valkonen, J., Kurki, P., Ivanova, E., Chirak, E., Korvigo, I. & Andronov, E. (2015). Comparative analysis of prokaryotic communities associated with organic and conventional farming systems. PLoS One, 10(12). doi: https://doi.org/10.1371/journal.pone.0145072

Bakker, M., Looft, T., Alt, D., Delate, K. & Cambardella, C. (2018). Bulk soil bacterial community structure and function respond to long-term organic and conventional agricultural management. Canadian journal of microbiology, 64(12), 901-914. doi: https://doi.org/10.1139/cjm-2018-0134

Gamboa, M. A., Laureano, S., & Bayman, P. (2003). Measuring diversity of endophytic fungi in leaf fragments: does size matter?. Mycopathologia, 156(1), 41-45. doi: https://doi.org/10.1023/A:1021362217723
Lu-Irving, P., Harenčár, J., Sounart, H., Welles, S., Swope, S., Baltrus, D. & Dlugosch, K. (2019). Native and Invading Yellow Starthistle (Centaurea solstitialis) Microbiomes Differ in Composition and Diversity of Bacteria. MSphere, 4(2). https://doi.org/10.1128/mSphere.00088-19

Jiao, J., Wang, H., Zeng, Y., & Shen, Y. (2006). Enrichment for microbes living in association with plant tissues. Journal of Applied Microbiology, 100(4), 830-837. https://doi.org/10.1111/j.1365-2672.2006.02830.x

Schmidt, J., Rodrigues, J., Brisson, V., Kent, A. & Gaudin, A. (2020). Impacts of directed evolution and soil management legacy on the maize rhizobiome. Soil Biology and Biochemistry, 145, 107794. doi: tps://doi.org/10.1016/j.soilbio.2020.107794

Finkel, O., Salas-González, I., Castrillo, G., Spaepen, S., Law, T., Teixeira, P., Jones, C., & Dangl, J. (2019). The effects of soil phosphorus content on plant microbiota are driven by the plant phosphate starvation response. PLOS Biology, 17(11), 1-34. https://doi.org/10.1371/journal.pbio.3000534

Yourstone, S., Lundberg, D., Dangl, J., & Jones, C.2014). MT-Toolbox: improved amplicon sequencing using molecule tags. BMC bioinformatics, 15(1), 1-7. doi: https://doi.org/10.1186/1471-2105-15-284

Joshi, N. & Sickle, F. (2011). No Title. A Sliding-Window, Adaptive, Quality-Based Trimming Tool for FastQ Files (Version 1.33). https://github.com/najoshi/sickle

Callahan, B., McMurdie, P., Rosen, M., Han, A., Johnson, A., & Holmes, S. (2016). DADA2: High-resolution sample inference from Illumina amplicon data. Nature Methods, 13(7), 581-583. https://doi.org/10.1038/nmeth.3869

Oksanen, J., Blanchet, F., Kindt, R., Legendre, P., Minchin, P., O"™hara, R. & Wagner, H. (2016). Vegan: Community Ecology Package. R Package Version. 2.0-10. CRAN. https://cran.r-project.org/soils FEMS microbiology ecology, 83(3), 607-621.

Salas, I. (2019). isaisg/ochibi. Github. https://github.com/isaisg/ohchibi

Chen, H. (2018). Package "VennDiagram". R Package Versión 1.6.20. CRAN. https://cran.r-project.org/

Kolde, R. (2019). pheatmap: Pretty Heatmaps. R Package Version 1.0.12. CRAN. https://cran.r-project.org/

Dos Santos, L. & Olivares, F. (2021). Plant microbiome structure and benefits for sustainable agriculture. Current Plant Biology, 100198. doi: https://doi.org/10.1016/j.cpb.2021.100198

Schlaeppi, K. & Bulgarelli, D. (2015). The plant microbiome at work. Molecular Plant-Microbe Interactions, 28(3), 212-217. doi: https://doi.org/10.1094/MPMI-10-14-0334-FI

Trivedi, P., Leach, J, Tringe, S., Sa, T. & Singh, B. (2020). Plant-microbiome interactions: From community assembly to plant health. Nature Reviews Microbiology, 18(11), 607-621. doi: https://doi.org/10.1038/s41579-020-0412-1

Longley, R., Noel, Z., Benucci, G., Chilvers, M., Trail, F. & Bonito, G. (2020). Crop Management Impacts the Soybean (Glycine max) Microbiome. Frontiers in Microbiology, 11, 1116. doi: https://doi.org/10.3389/fmicb.2020.01116

Miura, T., Sánchez, R., Castañeda, L., Godoy, K. & Barbosa, O. (2019). Shared and unique features of bacterial communities in native forest and vineyard phyllosphere. Ecology and evolution, 9(6), 3295-3305. doi: https://doi.org/10.1002/ece3.4949

Qu, Q., Zhang, Z., Peijnenburg, W., Liu, W., Lu, T., Hu, B. & Qian, H. (2020). Rhizosphere microbiome assembly and its impact on plant growth. Journal of agricultural and food chemistry, 68(18), 5024-5038. doi: https://doi.org/10.1021/acs.jafc.0c00073

Sun, A., Jiao, X., Chen, Q., Wu, A., Zheng, Y., Lin, Y. & Hu, H. (2021). Microbial communities in crop phyllosphere and root endosphere are more resistant than soil microbiota to fertilization. Soil Biology and Biochemistry, 153, 108113. doi: https://doi.org/10.1016/j.soilbio.2020.108113

Singh, U., Choudhary, A. & Sharma, S. (2020). Comparative performance of conservation agriculture vis-a-vis organic and conventional farming, in enhancing plant attributes and rhizospheric bacterial diversity in Cajanus cajan: A field study. European Journal of Soil Biology, 99, 103197. doi: https://doi.org/10.1016/j.ejsobi.2020.103197

Lupatini, M., Korthals, G., de Hollander, M., Janssens, T., y Kuramae, E. (2017). Soil microbiome is more heterogeneous in organic than in conventional farming system. Frontiers in microbiology, 7, 2064. doi: https://doi.org/10.3389/fmicb.2016.02064

Hartmann, M., Frey, B., Mayer, J., Mäder, P. & Widmer, F. (2015). Distinct soil microbial diversity under long-term organic and conventional farming. The ISME journal, 9(5), 1177-1194. doi: https://doi.org/10.1038/ismej.2014.210

Zhang, J., Bei, S., Li, B., Zhang, J., Christie, P. & Li, X. (2019). Organic fertilizer, but not heavy liming, enhances banana biomass, increases soil organic carbon and modifies soil microbiota. Applied Soil Ecology, 136, 67-79. doi: https://doi.org/10.1016/j.apsoil.2018.12.017

Zhang, M., Zhang, X., Zhang, L., Zeng, L., Liu, Y., Wang, X. & Ai, C. (2021). The stronger impact of inorganic nitrogen fertilization on soil bacterial community than organic fertilization in short-term condition. Geoderma, 382, 114752. doi: https://doi.org/10.1016/j.geoderma.2020.114752

Peruzzi, E., Franke-Whittle, I. H., Kelderer, M., Ciavatta, C. y Insam, H. (2017). Microbial indication of soil health in apple orchards affected by replant disease. Applied Soil Ecology, 119, 115-127. doi: https://doi.org/10.1016/j.apsoil.2017.06.003

Lupatini, M., Korthals, G., Roesch, L., & Kuramae, E. (2019). Long-term farming systems modulate multi-trophic responses. Science of the Total Environment, 646, 480-490. doi: https://doi.org/10.1016/j.scitotenv.2018.07.323

Aparna, K., Pasha, M., Rao, D. & Krishnaraj, P. (2014). Organic amendments as ecosystem engineers: microbial, biochemical and genomic evidence of soil health improvement in a tropical arid zone field site. Ecological engineering, 71, 268-277. doi: https://doi.org/10.1016/j.ecoleng.2014.07.016

Bengtsson, J., Ahnström, J. & Weibull, A. (2005). The effects of organic agriculture on biodiversity and abundance: a meta"analysis. Journal of applied ecology, 42(2), 261-269. doi: https://doi.org/10.1111/j.1365-2664.2005.01005.x

Tian, W., Wang, L., Li, Y., Zhuang, K., Li, G., Zhang, J.& Xi, Y. (2015). Responses of microbial activity, abundance, and community in wheat soil after three years of heavy fertilization with manure-based compost and inorganic nitrogen. Agriculture, Ecosystems & Environment, 213, 219-227. doi: https://doi.org/10.1016/j.agee.2015.08.009

Tian, W., Zhang, Z., Hu, X., Tian, R., Zhang, J., Xiao, X., & Xi, Y. (2015). Short-term changes in total heavy metal concentration and bacterial community composition after replicated and heavy application of pig manure-based compost in an organic vegetable production system. Biology and Fertility of Soils, 51(5), 593-603. doi: https://doi.org/10.1007/s00374-015-1005-4

Fierer, N., Bradford, M. A., & Jackson, R. B. (2007). Toward an ecological classification of soil bacteria. Ecology, 88(6), 1354-1364. doi: https://doi.org/10.1890/05-1839

Navarrete, A., Kuramae, E., de Hollander, M., Pijl, A., van Veen, J., & Tsai, S. M. (2013). Acidobacterial community responses to agricultural management of soybean in Amazon forest, 83 (3), 607-621. doi: https://doi.org/10.1111/1574-6941.12018

Bonanomi, G., De Filippis, F., Cesarano, G., La Storia, A., Ercolini, D. & Scala, F. (2016). Organic farming induces changes in soil microbiota that affect agro-ecosystem functions. Soil Biology and Biochemistry, 103, 327-336. doi: https://doi.org/10.1016/j.soilbio.2016.09.005

Banerjee, S., Kirkby, C., Schmutter, D., Bissett, A., Kirkegaard, J. & Richardson, A. (2016). Network analysis reveals functional redundancy and keystone taxa amongst bacterial and fungal communities during organic matter decomposition in an arable soil. Soil Biol. Biochem. 97, 188-198. doi: https://doi.org/10.1016/j. soilbio.2016.03.017

Kalam, S., Basu, A., Ahmad, I., Sayyed, R.., El Enshasy, H., Dailin, D. & Suriani, N. (2020). Recent understanding of soil Acidobacteria and their ecological significance: A critical review. Frontiers in Microbiology, 11, 2712. doi: https://doi.org/10.3389/fmicb.2020.580024

Touceda, M., Brader, G., Antonielli, L., Ravindran, V., Waldner, G., Friesl-Hanl, W. & Sessitsch, A. (2015). Combined amendment of immobilizers and the plant growth-promoting strain Burkholderia phytofirmans PsJN favours plant growth and reduces heavy metal uptake. Soil Biology and Biochemistry, 91, 140-150. doi: https://doi.org/10.1016/j.soilbio.2015.08.038

Teng, Y., Wang, X., Li, L., Li, Z., & Luo, Y. (2015). Rhizobia and their bio-partners as novel drivers for functional remediation in contaminated soils. Frontiers in plant science, 6, 32. doi: https://doi.org/10.3389/fpls.2015.00032

Ma, L., Geiser, D. M., Proctor, R. H., Rooney, A. P., O'Donnell, K., Trail, F. & Kazan, K. (2013). Fusarium pathogenomics. Annual review of microbiology, 67, 399-416. doi: https://doi.org/10.1146/annurev-micro-092412-155650

Wakelin, S., Warren, R., Harvey, P. & Ryder, M. (2004). Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biology and Fertility of Soils, 40(1), 36-43. doi: https://doi.org/10.1007/s00374-004-0750-6

Amaresan, N., Kumar, M., Annapurna, K., Kumar, K. & Sankaranarayanan, A. (Eds.). (2020). Beneficial Microbes in Agro-Ecology: Bacteria and Fungi. Estados Unidos: Elsevier. Recuperado de: https://elsevier.com/books-and-journals

Descargas

Los datos de descargas todavía no están disponibles.