Endophyte microbiome characterization in banana leaf and its variation in presence of Black Sigatoka pathogen (Pseudocercospora fijiensis)
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Keywords

Foliar
Leaf
Black Sigatoka
P. fijiensis
M. fijiensis
Microbiota
Endophytes

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How to Cite

Paladines-Montero, A., León-Reyes, A., Ramirez-Villacis, D. X., & Zapata-Ramón, C. G. (2022). Endophyte microbiome characterization in banana leaf and its variation in presence of Black Sigatoka pathogen (Pseudocercospora fijiensis). ACI Avances En Ciencias E Ingenierías, 14(1), 16. https://doi.org/10.18272/aci.v14i1.2299

Abstract

We describe the bacterial and fungal microbiome of the banana (Musa x paradisiaca) leaf in healthy and necrotic phases of Black Sigatoka or black leaf streak disease (Pseudocercospora fijiensis), evaluated under organic and conventional agronomic management in El Oro province, Ecuador. Samples were collected for subsequent DNA sequencing and analysis of the 16S (V3-V4) and ITS markers. This study suggests that the fungal microbiome from the organic farming system decreases its diversity due to pathogen's presence while diversity increases upon conventional practices. Also, we observed that bacteria from the genus Pseudomonas fluorescens was more abundant in the healthy organic leaf, a known beneficial microorganism for plants. The banana"™s phyllosphere endophyte microbiome depends on the cultivation system, and diseased conditions significantly change the microbiome in the presence of black leaf streak disease.

Key words: necrotic, sequencing, diversity, ASV, phyllosphere

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Copyright (c) 2022 Alejandra Paladines-Montero, Antonio León-Reyes, Dario X Ramirez-Villacis, Claudia G. Zapata-Ramón