Mushrooms feed on organic matter, many of them with high nutritional value and poorly used. Large-scale crops require a strong economic investment to control temperature and humidity variables. The overall aim of this study is to cultivate Pleurotus ostreatus by hand without further automated control of temperature and humidity, optimizing the use of agro-industrial residues: rice husk (Oriza sativa L.), coffee husk (Coffea arabica L.) and sawdust (sawn wood particles), as substrates in the production of this species. Determining production rate (TP), biological efficiency (EB), and the percentage of protein. The results indicate that the combination of substrates, rice husk + coffee husk + sawdust (T1) was better in production rate (80.20%) and biological efficiency (15.23%) over only a sawdust substrate (T2). The percentage of protein 40% (T2) and 35% (T1) were not significantly different. The cultivation of P. ostreatus is shown as a sustainable alternative source of protein in the diet of vulnerable populations.
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