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ACI Avances en Ciencias e Ingenierías

SECTION B: LIFE SCIENCES

Vol. 7 No. 2 (2015)

Antimicrobial resistance within serogroups of <em>Shigellae</em> isolates in the city of Quito Ecuador

DOI
https://doi.org/10.18272/aci.v7i2.255
Submitted
January 22, 2016
Published
2015-12-30

Abstract

The pathotype Shigellae includes bacillus from the species S. flexneri, S. sonnei, S. boydii and S. dysenteriae which are causatives of the disease known as bacillary dysentery or shigellosis. The pathogenicity of the genus is characterized by the presence of high antibiotic resistance. The aim of this study was to identify the species and antimicrobial susceptibility in clinical isolates of Shigellae. We analyzed 79 isolates obtained from Zurita & Zurita Laboratorios and Vozandes Hospital in the city of Quito. Three species were obtained by serotyping, using polyclonal antisera: S. flexneri (n=50; 63,29%), S. sonnei (n=23; 29,11%), and S. boydii (n=6; 7,59%). The antimicrobial susceptibility was analyzed using the Kirby Bauer method and recommendations from the “Clinical and Laboratory Standards Institute” (CLSI). Most of the isolates showed resistance to tetracycline (n=76; 96,20%), ampicillin (n=75; 94,94%), trimethoprim/sulfamethoxazole (n=68; 86,08%) and chloramphenicol (n=67; 84,81%). The results showed the incidence of multidrug resistance to antibiotics commonly used for treating shigellosis, and the presence of three species of Shigellae.

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References

  1. Murray, P.; Rosenthal, K.; Pfaller, M. 2013. "Medical Microbiology". Elsevier Inc: Philadelphia.
  2. Germani, Y.; Sansonetti, P 2006. "The Genus Shigella" en: "The Prokaryotes. A handbook on the Biology of Bacteria. 6. Proteobacteria: Gamma Subclass", M. Dworkin, S. Falkow, E. Rosenberg, K. Schleifer, E. Stackebrandt, Springer, New York: 99-116.
  3. Ye, C.; Lan, R.; Xia, S.; Zhang, J.; Sun, Q.; Zhang, S.; Jing, H.; Wang, L.; Li, Z.; Zhou, Z.; Zhao, A.; Cui, Z.; Cao, J.; Jin, D.; Huang, L.; Wang, Y.; Luo, X.; Bai, X.; Wang, Y.; Wang, P; Xu, Q.; Xu, J. 2010. "Emergence of a new resistant serotype X variant in an epidemic clone of Shigella flexneri". Journal of Clinical Microbiology, 48: 419-426.
  4. Kotloff, K. L.; Winickoff, J. P; Ivanoff, B.; Clemens, J. D.; Swerdlow, D. L.; Sansonetti. P J.; Adak, G. K.; Levine; M. M. 1999. "Global burden of Shigella infections: implications for vaccine development and implementation of control strategies". Bulletin of the World Health Organization, 77: 651-666.
  5. Carrera, M. S.; Yuga, J. C. 2012. "Anuario de Estadísticas Hospitalarias Camas y Egresos 2012". Instituto Nacional de Estadística y Censos: Quito, Ecuador. http://www.ecuadorencifras.gob.ec/documentos/web-inec/Estadisticas_Sociales/Camas_Egresos_Hospitalarios/Publicaciones-Cam_Egre_Host/Anuario_Camas_Egresos_Hospitalarios_2012.pdf.
  6. Seidlein, L.; Kim, D. R.; Ali, M.; Lee, H.; Wang, X. Y; Thiem, V D.; Canh, D. G.; Chaicumpa, W.; Agtini, M. D.; Hossain, A.; Bhutta, Z. A.; Mason, C.; Sethabutr, O.; Talukder, K.; Nair, G. B.; Dee, J. L.; Kotloff, K.; Clemens, J. 2006. "A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology". PLoS Medicine, 3 (9): 1556-1569.
  7. Gu, B.; Cao, Y.; Pan, S.; Zhuang, L.; Yu, R.; Peng, Z.; Qian, H.; Wei, Y.; Zhao, L.; Liu, X.; Tong, M. 2012. "Comparison of the prevalence and changing gesistance to nalidixic acid and ciprofloxacin of Shigella between Europe-America and Asia-Africa from 1998 to 2009". International Journal of Antimicrobial Agents, 40: 9-17.
  8. Centers for Disease Control and Prevention. CDC. 2014. "Health Information for International Travel 2014". Oxford University Press: New York.
  9. Edwards, P; Edwing, W. 1972. "Identification of Enterobacteriaceae". Burgers Publ. Co.: Minneapolis.
  10. Difco. 2011. "Manual Difco Shigella Antisera Poly O". Becton, Dickinson and Company: County Clare: 11-13.
  11. Bauer, A. W.; Kirby, W. N.; Sherrins, G. J.; Turk, M. 1966. "Antibiotic susceptibility testing by standardized single disk method". American Journal of Clinical Pathology, 45: 493-496.
  12. Clinical and Laboratory Standards Institute. CLSI. 2010. "Performance standards for antimicrobial susceptibility testing; Sixteen International Supplement, CLSI document". Clinical and Laboratory Standards Institute: Pennsylvania.
  13. Organización Panamericana de la Salud. 2009. "Informe Anual de la Red de Monitoreo/Vigilancia de la Resistencia a los Antibióticos". OPS: Basilia.
  14. Instituto Nacional de Estadísticas y Censos. INEC. 2009. "Anuario de Estadísticas Hospitalarias: Camas y Egresos. Diez principales causas de morbilidad. Lista Internacional Detallada-CIE-1015". INEC: Quito.
  15. Gonzales, S.; Cecchini, D. 2003. "Diagnóstico e investigación epidemiológica contra las enfermedades trasmitidas por alimentos". OPS: Buenos Aires.
  16. Guerrant, R. L.; Hughes, J. M.; Lima, J. 1999. "Diarrhea in developing countries: magnitude, special setting and etiologies". Journal ofInfectious Diseases, 1: 41.
  17. Von Seidlein, L; Kim, D. R; Ali, M.; Lee, H.; Wang, X.; Thiem, V D.; Canh do, G.; Chaicumpa, W.; Agtini, M. D.; Hossain, A.; Bhutta, Z. A.; Mason, C.; Sethabutr, O.; Talukder, K.; Nair, G. B.; Deen, J. L.;, Kotloff, K.; Clemens, J. 2006. "A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology". PLoS Medicine, 3 (9): 1556-1569.
  18. Wei, H. L.; Wang, Y. W.; Li, C. C.; Tung, S. K.; Chiou, C. S. 2007. "Epidemiology and evolution of genotype and antimicrobial resistance of an imported Shigella sonnei clone circulating in central Taiwan". Diagnostic Microbiology and Infectious Diseases, 58: 469-475.
  19. Mota, I.; Varela, G.; Gadea, M.; Caffer, M.; Sirok, A.; Schelotto, F. 2005. "Serotipos, perfil plasmídico y anti-biotipos de cepas de Shigella flexneri aisladas de niños menores de 5 años con diarrea sanguinolenta usuarios de los servicios de Salud pública". Revista Médica de Uruguay, 21: 30-36.
  20. Merino, A.; Hreñuk, E.; Ronconi, M.; Alonso, M. 2004. "Resistencia a antibióticos y epidemiología molecular de Shigella spp. en el nordeste argentino". Revista Panamericana de Salud Pública, 15 (4): 219-224.
  21. Ramírez, M.; Valdés, N.; Bravo, L.; Fernández, A.; Castañeda, N. 2004. "Perfil plasmídico resistencia antimicrobiana en cepas de Shigella aisladas en Cuba". Revista Cubana de Medicina Tropical, 56 (3): 178-185.
  22. Silva, T.; Nogueira, A.; Magalhaes. F.; Fagundes, A.; Pereira, L.; Puccinelli, P. 2008. "Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (western Amazon region), Brazil". Memorias del Instituto Osxaldo Cruz, Rio de Janeiro, 103 (7): 731-733.
  23. Xing, K.; Bing, G.; Shiyang, P.; Mingqing, T. 2011. "Epidemiology and molecular mechanism of integron-mediated antibiotic resistance in Shigella". Arceives of Microbiology, 193: 767-774.
  24. Qu, M.; Zhang, X.; Liu, G.; Huang, Y.; Jia, L.; Liang, W.; Li, X.; Wu, X.; Li, J.; Yan, H.; Kan, B.; Wang, Q. 2014. "An eight-year study of Shigella species in Beijing, China: serodiversity, virulence genes, and antimicrobial resistance". The Journal of Infection in Developing countries, 8 (7): 904-908.
  25. Boheme, C.; Iglesias, T.; Loyola, A.; Soto, L.; Rodríguez, G.; Reydet, P. 2002. "Comparación de la susceptibilidad de especies de Shigella a antimicrobianos de uso habitual en el Hospital Regional de Temuco, Chile". Revista Médica de Chile, 130 (9): 1021-1026.
  26. Vila, J.; Gascón, J.; Abadía, S.; Gómez, J.; Marco, F.; Moreno, A. 1994. "Antimicrobial resistance of Shigella isolates causing traveler"™s diarrhea". Antimicrobiological Agents Chemotherapy, 38 (11): 2668-70.
  27. Bassa, A.; Dadie, A.; Guessennd, N.; Gbonon, V; Dako, E.; Dje, M.; Dosso, M. 2010. "Virulence Factors and Resistance Profile of Shigella Isolated During Infectious Diarrhea in Abidjan, Cote D"™Ivoire". Journal of Applied Sciences Research, 6 (6): 594-599.
  28. Zurita, J. 2010. "Shigella spp." en "Resistencia bacteriana en el Ecuador". Centro de Publicaciones PUCE: Quito: 55-57.
  29. Replogle, M.; Fleming, D.; Cieslak. O. 2000. "Emergence of Antimicrobial Resistance Shigellosis in Oregon". Clinical Infectious Diseases, 30: 515-519.
  30. Saurina, G.; Quale, J. M.; Manikal, V M. 2000. "Antimicrobial resistance in Enterobacteriaceae in Brooklyn, NY: epidemiology and relation to antibiotic usage patterns". Journal Antimicrobial Chemotherapy, 45: 895-898.