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ACI Avances en Ciencias e Ingenierías

Bionucleares 2020

Vol. 12 No. 3 (2020): Aplicaciones Nucleares (2021)

Dosimetry and medium lethal dose (LD50) for gamma rays induced mutations in two barley varieties (Hordeum Vulgare L.)

DOI
https://doi.org/10.18272/aci.v12i3.2019
Submitted
October 4, 2020
Published
2021-05-05

Abstract

Barley is a crop of economic, social and food importance in the Ecuadorian highlands. It´s the second most cultivated cereal in Ecuador and the fourth in the world. Induced mutation is an improvement technique that allows generate genetic variability; that´s why, INIAP is applying this technique in the barley crop in search of germplasm with desirable characteristics. The main objective of this research was determine the dosimetry and medium lethal dose of irradiation to induce mutations in barley varieties, using five doses of gamma rays (0, 100, 150, 200 and 250 Gy). The parameters evaluated were: germination (in greenhouse); emergency, seedling height and vigour (in the field). The results showed that the varieties responded in different ways to the distinct doses used, however, it was evident that as the dose increased, the emergency, seedling height and vigor were affected and diminished. The medium lethal dose was determined to be between 150 and 200 Gy. However, the dose that produced slight morphological changes in the materials and is considered like the optimal dose is 150 Gy.

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