Optical aptasensor for in situ detection and quantification of methylxanthines in Ilex guayusa
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Publicado:
abr 27, 2022
Palabras clave:
aptasensor, aptámero, metilxantinas, cafeína, teofilina, Ilex guayusa
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Resumen
El presente trabajo buscó el desarrollo de un sistema para detectar y cuantificar metilxantinas en Ilex guayusa. El sistema, denominado IPMA por sus siglas en inglés (Aptasensor de Metabolitos de Plantas In situ), se basa en un aptasensor óptico que integra un complejo de ADN y una porfirina (NMM IX). Se evaluó la capacidad de IPMA para detectar cantidades conocidas de teofilina y cafeína tanto en solución como infiltradas en hojas de guayusa. Los límites de detección determinados fueron: 0.25 mM para teofilina en solución, 0.1 mM para cafeína en solución, 130 mM para cafeína en hojas de I. guayusa. Estos resultados demuestran el potencial de IPMA para detectar y cuantificar metabolitos de interés directamente de muestras biológicas. El desarrollo de este tipo de herramienta ofrece una amplia gama de aplicaciones como la determinación in situ de estrés fisiológico en plantas y la caracterización de variedades vegetales con mayor contenido de compuestos de interés farmacéutico o alimentario.
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León Intriago, B. A., Montero Oleas, A. C., Mollocana Yánez, D. S., Calderón Carvajal, D. J., & Torres Proaño, M. de L. (2022). Optical aptasensor for in situ detection and quantification of methylxanthines in Ilex guayusa . ACI Avances En Ciencias E Ingenierías, 14(1). https://doi.org/10.18272/aci.v14i1.2301
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SECCIÓN B: CIENCIAS BIOLÓGICAS Y AMBIENTALES
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Citas
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9. Feng, C., Dai, S., & Lei, W. (2014). Optical Aptasensors for Quantitative Detection of Small Biomolecules: A Review. Biosensors and Bioelectronics, 59, 64-74. doi: http://doi.org/10.1016/j.bios.2014.03.014
10. Hernández, J., & Botero Hincapié, J. A. (2012). Aptámeros: Agentes Diagnósticos y Terapéuticos. Iatreia, 25(2), 159-168. ISSN 0121-0793. https://www.redalyc.org/articulo.oa?id=180523365008
11. Hong, P., Li, W., & Li, J. (2012). Applications of Aptasensors in Clinical Diagnostics. Sensors. Sensors (Basel), 12(2), 1181–1193. doi: https://doi.org/10.3390/s120201181
12. Sett, A., Das, S., Sharma, P., & Bora, U. (2012). Aptasensors in Health, Environment and Food Safety Monitoring. Open Journal of Applied Biosensor, 1(2). doi: https://doi.org/10.4236/ojab.2012.12002
13. Rana, J. S., Jindal, J., Beniwal, V., & Chhokar, V. (2010). Utility Biosensors for Applications in Agriculture – A Review. Journal of American Science, 6(9), 353-375. https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.469.8344&rep=rep1&type=pdf
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15. Pagare, S., Manila, B., Tripathi, N., & Bansal, Y. K. (2015). Secondary Metabolites of Plants and Their Role: Overview. Current Trends in Biotechnology and Pharmacy, 9(3), 293-304. ISSN 2230-7303 (Online).
16. Andreeva, E. Y., Dmitrienko, S. G., Zolotov, Y. A. (2012) Methylxanthines: properties and determination in various objects Russian Chemical Reviews, 81(5), 397-414. doi: http://dx.doi.org/10.1070/RC2012v081n05ABEH004220
17. Evans, J., Richards, J. R., & Battisti, A. S. (2021) Caffeine. In: StatPearls. StatPearls Publishing.
18. Bucklin, M. H., & Groth, C. M. (2014). Theophylline. In Encyclopedia of Toxicology (Third Edition). Elsevier. ISBN: 978-0-12-386455-0
19. Ashihara, H., Kato, M., & Crozier, A. (2011). Distribution, biosynthesis and catabolism of methylxanthines in plants. Handb. Exp. Pharmacol., 200, 11-31. doi: http://doi.org/10.1007/978-3-642-13443-2_2
20. Kapp, R., Mendes, O., Roy, S., & McQuate, R. (2016). General and Genetic Toxicology of Guayusa Concentrate (Ilex guayusa). International Journal of Toxicology, 35(2), 222-242. doi: https://doi.org/10.1177/1091581815625594
21. Rätsch, C. (2005). The encyclopedia of psychoactive plants: Ethnopharmacology and its applications. Simon and Schuster.
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23. Rankin, C. J., Fuller, E. N., Hamor, K. H., Gabarra, S. A., & Shields, T. P. (2006). A Simple Fluorescent Biosensor for Theophylline Based on Its RNA Aptamer. Nucleosides, Nucleotides and Nucleic Acids, 25(2), 1407-1424. doi: http://doi.org/10.1080/15257770600919084
24. Rivera, P. (2016). Diseño de un sistema de reporte, específico, in vitro de un metabolito de interés agroindustrial, teofilina, vía ensayos con aptámeros y fluorescencia (Thesis). Universidad San Francisco de Quito USFQ.
25. Yett, A., Yingqi, L., Beseiso, D., Miao, J., & Yatsunyk., L. A. (2019). N-Methyl Mesoporphyrin IX as a Highly Selective Light-up Probe for G-Quadruplex DNA. Journal of Porphyrins and Phthalocyanines, 23(11n12), 1195-1215. doi: https://doi.org/0.1142/S1088424619300179
26. Schack, J. A., & Waxler, S. H. (1949). An Ultraviolet Spectrophotometric Method for the Determination of Theophylline and Theobromine in Blood and Tissues. The Journal of Pharmacology and Experimental Therapeutics, 97(3), 283-91. https://pubmed.ncbi.nlm.nih.gov/15392550/
27. Londoño-Larrea, P., Zapata, S., Lara-Lopez, M., & Villamarin-Barriga, E. (2018). Preliminary study of caffeine extraction from Ilex guayusa L. leaves using supercritical carbon dioxide. Conference: MOL2NET 2018, International Conference on Multidisciplinary Sciences (4th edition). doi: https://doi.org/10.3390/mol2net-04-05297
28. Miller, J. N., & Miller, J. C. (2010). Statistics for analytical chemistry: 6th ed. Pearson Education Limited.
29. Schack, J. A., & Waxler, S. H. (1949). An Ultraviolet Spectrophotometric Method for the Determination of Theophylline and Theobromine in Blood and Tissues. The Journal of Pharmacology and Experimental Therapeutics, 97(3), 283-91. https://pubmed.ncbi.nlm.nih.gov/15392550/
30. Umar, M. I., Ji, D., Chan, C. Y., & Kwok, C. K. (2019). G-Quadruplex-Based Fluorescent Turn-On Ligands and Aptamers: From Development to Applications. Molecules, 24(13), 2416. doi: https://doi.org/10.3390/molecules24132416
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