Vol. 2 No. 2 (2010), SECTION B: LIFE SCIENCES
Vol. 2 No. 2 (2010)

Propagation of Tamarillo (Solanum betaceum) via somatic embryogenesis

SECTION B: LIFE SCIENCES
Published 2010-06-01
Venancio S. Arahana B.
Universidad San Francisco de Quito
Antonio R. Cabrera V.
Universidad San Francisco de Quito
María de Lourdes Torres P.
Universidad San Francisco de Quito
Portada Avances en Ciencias e Ingenierías Volumen 2 - Número 2 2010
PDF (Spanish)

Keywords

Somatic embryogenesis
Solanum betaceum
sucrose
NAA (a-napthaleneacetic acid)
somatic embryo

How to Cite

Arahana B., V. S., Cabrera V., A. R., & Torres P., M. de L. (2010). Propagation of Tamarillo (Solanum betaceum) via somatic embryogenesis. ACI Avances En Ciencias E Ingenierías, 2(2). https://doi.org/10.18272/aci.v2i2.28
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

A reproducible micropropagation protocol for tamarillo (Solanum betaceum) via somatic embryogenesis was established in this study. Three types of explants were assayed: leaves, hypocotyls and zygotic embryos; all of them obtained from in vitro cultured seeds. Different concentrations of auxins 2, 4-D (2,4-dichlorophenoxyacetic acid) and NAA (a- napthaleneacetic acid) and sucrose were tested to find out an appropriate medium that sti­mulated the formation of embryogenic calli. MS medium supplemented with 5 mg/L of NAA and 90 g/L of sucrose (pH 5.8) induced somatic embryos formation from the upper thirds of hypocotyls and from zygotic embryos. One-hundred percent of the explants deri­ved from zygotic embryos and hypocotyls developed big whitish calli. However, only 40 % of the hypocotyl-derived calli and 70 % of the zygotic embryo-derived calli produced so­matic embryos. The results showed that somatic embryos were obtained after a four-week incubation period in MS medium supplemented with 5 mg/L of NAA and 90 g/L of sucrose. The somatic embryos grew rapidly and produced plantlets with normal phenotypes. These results suggest that this technique could be applied for large scale production of Tamarillo and other agronomically important Andean fruit crops.

PDF (Spanish)

References

Soria, N. 2005. "Tomate de árbol/tamarillo/sweet tomato." Technical report. Servicio de Información Agropecuaria del Ministerio de Agricultura y Ganadería del Ecuador.

Lebn, J. 1996. "Guía para el cultivo de tomate de árbol." Technical report. INIAP-COTESUP.

Cadena, E. 2006. "Estudio de prefactibilidad para el tomate de árbol." Technical report. SICA, Ministerio de Agricultura y Ganadería del Ecuador.

Kreuter, M. L. 2006. "Andean fruits and vegetables of the world." Technical report. MNS, European Fruits and Vegetals Reports.

Hernández, L. and Carneros, E. 2005. "Embriogénesis somática como elemento de la biotecnología forestal." Technical report. Instituto Madrileño de Investigación y Desarrollo Rural (IMIDRA).

Canhoto, J. M. and Lopes, M. L. 2005. "Protocol of somatic embryogenesis: Tamarillo (Cyphomandra beta-cea)." Technical report. Facultad de Ciencias e Tecnologia. Universidade de Coimbra.

López-Vela, D. 2005. "Embriogénesis somática en Solanum tuberosum" Technical report. L. Cv Andinita. Instituto Madrileño de Investigación y Desarrollo Rural (IMIDRA).

Murashige, T. and Skoog, F. 1962. "A revised medium for rapid growth and bioassays with tobacco tissue cultures." Physiol. Plant. 15,473-497.

Jiménez, V and Guevara, E. 1995. "Regeneración in vitro mediante embriogénesis somática de variedades de cítricos." Technical report. Centro de Investigaciones Agronómicas. Universidad de Costa Rica. San José de Costa Rica.

Márquez, M. Sánchez, R. 2003. "Factores que afectan la obtención de embriones somáticos blanco-opacos de aguacate." Technical report. Dpto. de Biología Vegetal. Universidad de Málaga. Málaga, España.

Merkle, S. A. and Parrot, B. S. 1995. "Morphogenic aspects of somatic embryogenesis." In Vitro Embryogenesis in Planta. 155-203.

Gomez, R. 1998. "Generalidades sobre la embriogénesis somática." Technical report. Resúmenes del curso Internacional de Propagación in vitro de especies vegetales. Santa Clara, Cuba.

Copyright notice

Authors who publish in the journal ACI Avances en Ciencias e Ingenierías accept the following terms:

  1. The authors will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the Creative Commons Attribution License that allows third parties to share the work provided that its author and its first publication in this journal is indicated.
  2. Authors may adopt other non-exclusive license agreements for the distribution of the published version of the work, thereby being able to publish it in a monographic volume or reproduce it in other ways, provided that the initial publication in this journal is indicated.
  3. Authors are permitted and advised to disseminate their work over the Internet:
    1. Before submission to the journal, authors can deposit the manuscript in pre-publication files/repositories (preprint servers/repositories), including arXiv, bioRxiv, figshare, PeerJ Preprints, SSRN, and others, which can produce interesting exchanges and increase citations of the published work (see The effect of open access).
    2. After submission, it is recommended that authors deposit their article in their institutional repository, personal website, or scientific social network (such as Zenodo, ResearchGate or edu).